(KO Validated) BAX Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1580 / ￥2520 / ￥4020 |
货号：E-AB-63606
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | BCL2L4,BAX,BAX抗体 |
| Swissprot | Q07812 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human BAX (NP_620116.1). |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,, 1:500-1:2000;IHC,, 1:100-1:200; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Background | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. |
Images
Western blot analysis of extracts of various cell lines using BAX Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and BAX knockout (KO) 293T cells using BAX Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat ovary using BAX Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using BAX Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human breast cancer using BAX Polyclonal Antibody at dilution of 1:100 (40x lens).
