AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]
| 规格： | / 50μg / 500μg / 1mg |
| 价格： | / ￥600 / ￥1960 / ￥3560 |
货号：E-AB-F10180
货期：咨询
宿主: Rat
反应性: M
应用: FCM
Overview
| Synonyms | CD54;Icam-1;Icam1;Intercellular adhesion molecule 1;MALA-2;MyD10 |
| Swissprot | P13597 |
| Source | Rat |
| Reactivity | Mouse |
| Immunogen | Recombinant Mouse CD54 protein |
| Application | FCM |
| Recommended dilution | FCM,,2 μg/mL(1×10^5-5×10^5 cells) |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Clone No | YN1/1.7.4 |
|---|---|
| Isotype | Rat IgG2b, κ |
| Purification | >98%, Protein A/G purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS. < 1.0 EU per mg of the antibody as determined by the LAL method |
| Background | CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues. |
