AFP Polyclonal Antibody
| 规格： | / 50μL / 100μL |
| 价格： | / ￥1010 / ￥1640 |
货号：E-AB-40293
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | Alpha-fetoprotein,Alpha-1-fetoprotein,Alpha-fetoglobulin,Afp |
| Swissprot | P02772 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Mouse Afp protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:1000;IHC,,1:200-1:500;IF,,1:100-1:200; |
| Concentration | 1.1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Secreted |
| Tissue specificity | Plasma.Highest expression level in embryo. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
Images
Western blot with Afp Polyclonal antibody at dilution of 1:1000.lane 1:Hep G2 whole cell lysate,lane 2:Mouse placenta,lane 3:Rat placenta,lane 4:Mouse Fetal liver,lane 5:Rat Fetal liver,lane 6: Mouse liver,lane 7:Mouse kidney,lane 8:Rat liver,lane 9:Rat kidney
Immunohistochemistry of paraffin-embedded Mouse liver using AFP Polyclonal Antibody at dilution of 1:50
