ALKBH8 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-52350
宿主: Rabbit
反应性: H
应用: WB,IHC
Overview
| Synonyms | 4930562C03Rik,8030431D03Rik,9430088N01Rik,ABH8,alkB alkylation repair homolog 8 (E. coli),alkB alkylation repair homolog 8,AlkB homologue 8,ALKB8,ALKBH8,Alkylated DNA repair protein alkB homolog 8,FLJ38204,MGC10235,OTTHUMP00000211286,OTTHUMP00000211287,Probable alpha-ketoglutarate-dependent dioxygenase ABH8,RGD1304687,S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8,tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8,ALKBH8抗体 |
| Swissprot | Q96BT7 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Full length fusion protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:25-1:100; |
| Concentration | 0.6mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Predominantly cytoplasmic. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene. |
Images
Western blot analysis of A549 and Jurkat cell lysates using ALKBH8 Polyclonal Antibody at dilution of 1:300
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using ALKBH8 Polyclonal Antibody at dilution of 1:25(×200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ALKBH8 Polyclonal Antibody at dilution of 1:25(×200)
