AMPK alpha2 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-40256
宿主: Rabbit
反应性: H,R
应用: WB,IHC,IF
Overview
| Synonyms | 5'-AMP-activated protein kinase catalytic subunit alpha-2,AAPK2,ACACA kinase,Acetyl-CoA carboxylase kinase,AMPK alpha 2 chain,AMPK subunit alpha-2,AMPK2,AMPKa2,AMPKalpha2,HMGCR kinase,Hydroxymethylglutaryl-CoA reductase kinase,PRKAA,PRKAA2,Protein kinase AMP activated alpha 2 catalytic subunit,Protein kinase AMP activated catalytic subunit alpha 2,AMPK alpha2抗体 |
| Swissprot | Q09137 |
| Source | Rabbit |
| Reactivity | Human,Rat |
| Immunogen | Recombinant Rat 5'-AMP-activated protein kinase catalytic subunit alpha-2 protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB 1:500-1:2000 IHC 1:100-1:200 IF 1:100-1:400 |
| Concentration | 1mg/mL |
Properties
| Cellular localization | Cytoplasm. Nucleus. |
| Tissue specificity | Skeletal muscle, lower levels in liver, heart and kidney. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
| Background | The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. |
Images
Western Blot analysis of Rat heart and Rat muscle using AMPK alpha2 Polyclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Human breast using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of McF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
Immunofluorescence analysis of MCF7 cells using AMPK alpha2 Polyclonal Antibody at dilution of 1:100
