APEX1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-13896
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | AP endonuclease 1,AP endonuclease class I,AP lyase,APE 1,APE,APE-1,APEN,APEX 1,APEX,APEX nuclease (multifunctional DNA repair enzyme) 1,Apex nuclease 1,APEX nuclease,APEX1,APEX1,Apurinic endonuclease,Apurinic-apyrimidinic endonuclease 1,Apurinic/apyrimidinic (abasic) endonuclease,Apurinic/apyrimidinic endonuclease 1,Apurinic/apyrimidinic exonuclease,APX,BAP1,Deoxyribonuclease (apurinic or apyrimidinic),DNA (apurinic or apyrimidinic site) lyase,DNA-(apurinic or apyrimidinic site) lyase,mitochondrial,EC 4.2.99.18,HAP 1,HAP1,Human Apurinic endonuclease 1,MGC139790,Multifunctional DNA repair enzyme,Redox factor 1,Redox factor-1,REF 1,REF 1 protein,REF-1,REF1,REF1 protein,APEX1抗体 |
| Swissprot | P27695 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human APEX1 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000; |
| Concentration | 0.2 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Mitochondrion. The cleaved APEX2 is only detected in mitochondria (By similarity). Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A. Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress and Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. |
Images
Western Blot analysis of Hela cell and Human liver cancer tissue, Raji, Jurkat, 293T and PC3 cell using APEX1 Polyclonal Antibody at dilution of 1:450
Immunohistochemistry of paraffin-embedded Human colon cancer using APEX1 Polyclonal Antibody at dilution of 1:20
Immunohistochemistry of paraffin-embedded Human lung cancer using APEX1 Polyclonal Antibody at dilution of 1:20
