ASAH2 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-14747
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | Acylsphingosine deacylase 2,AI585898,ASAH2,ASAH2,BCDase,hCD,HNAC1,LCDase,MGC129777,mitochondrial ceramidase,N acylsphingosine amidohydrolase (non lysosomal ceramidase) 2,N acylsphingosine amidohydrolase 2,N CDase,N-acylsphingosine amidohydrolase 2,N-CDase,NCDase,Neutral ceramidase soluble form,Non lysosomal ceramidase,Non-lysosomal ceramidase,ASAH2抗体 |
| Swissprot | Q9NR71 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human ASAH2 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:200-1:1000;IHC,,1:25-1:100; |
| Concentration | 0.7 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cell membrane. The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo. |
| Tissue specificity | Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. |
Images
Western Blot analysis of Lovo cell and Mouse heart tissue using ASAH2 Polyclonal Antibody at dilution of 1:270
Immunohistochemistry of paraffin-embedded Human brain using ASAH2 Polyclonal Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Human colon cancer using ASAH2 Polyclonal Antibody at dilution of 1:50
