BAG3 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-18208
宿主: Rabbit
反应性: H,M
应用: WB,IHC
Overview
| Synonyms | BAG 3,BAG family molecular chaperone regulator 3,BAG-3,Bag3,BAG3,Bcl 2 binding protein,Bcl-2-associated athanogene 3,Bcl-2-binding protein Bis,BCL2 associated athanogene 3,BCL2 binding athanogene 3,BIS,CAIR 1,Docking protein CAIR 1,Docking protein CAIR-1,MFM6,BAG3抗体 |
| Swissprot | O95817 |
| Source | Rabbit |
| Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human BAG3 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:40-1:200; |
| Concentration | 0.6mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytosol,Nucleus,Plasma Membrane,Other locations:cytoplasm,neuron projection,Z disc |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. |
Images
Western blot analysis of Mouse heart tissue lysate using BAG3 Polyclonal Antibody at dilution of 1:250
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using BAG3 Polyclonal Antibody at dilution of 1:35(×200)
Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using BAG3 Polyclonal Antibody at dilution of 1:35(×200)
