beta Tubulin Monoclonal Antibody
| 规格： | / 50μL / 100μL |
| 价格： | / ￥499 / ￥800 |
货号：E-AB-48019
宿主: Mouse
反应性: H, M, E, S, Z
应用: WB, IF
Overview
| Synonyms | Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, TUBB 1, TUBB 2, TUBB 5, TUBB, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain |
| Swissprot | P99024 |
| Source | Mouse |
| Reactivity | Human, Mouse, E.coli, Scallop, Zebrafish |
| Immunogen | Recombinant human TUBB protein |
| Application | WB(Detection kit: E-IR-R304), IF |
| Recommended dilution | WB,,1:5000-10000;IF,,1:2000-4000; |
| Concentration | 3mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm, Cytoskeleton, Microtubule |
| Clone No | 3A7 |
|---|---|
| Isotype | IgG2a |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot. |
Images
Western blotting with anti-Beta Tubulin monoclonal antibody at dilution of 1:5000.
Lane 1: Hela cell lysates, Lane 2: Caco-2 cell lysates, Lane 3: HT-29 cell lysates, Lane 4: NIH/3T3 cell lysates, Lane 5:Raw264.7 cell lysates, Lane 6: Rat liver
Immunofluorescent analysis of HEK293 cells with anti-beta Tubulin monoclonal antibody at dilution of 1:4000.
