Brdu Monoclonal Antibody
| 规格： | / 60μL / 120μL |
| 价格： | / ￥1420 / ￥2310 |
货号：E-AB-70248
宿主: Mouse
反应性: H,M,R
应用: IHC,IF
Overview
| Synonyms | Bromodeoxyuridine;Bromodeoxyuridine;5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.,Brdu抗体 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat |
| Immunogen | KLH conjugated BrdU |
| Application | IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | IHC,,1:100-1:500;IF,,1:100-1:500; |
| Concentration | 0.7 mg/mL |
| Clonality | Monoclonal |
Properties
| Clone No | 4B6C12 |
|---|---|
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. |
Images
Immunohistochemistry analysis of paraffin-embedded mouse jejunum(intraperitoneal injection BrdU every 2 hours for 4 times) using Brdu Monoclonal Antibody at dilution of 1:300.
Immunofluorescence analysis of Hela cells（treated with 0.03mg/ml BrdU for 40 min ) using Brdu Monoclonal Antibody at dilution of 1:100.
