CA9 Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22034
宿主: Mouse
反应性: H
应用: WB,IHC-p,IF
Overview
| Synonyms | CA-IX,CA9,CAH9,CAIX,Carbonate dehydratase IX,Carbonic anhydrase 9,Carbonic anhydrase IX,Carbonic dehydratase,G250,Membrane antigen MN,MN,P54/58N,pMW1,RCC associated protein G250,RCC-associated antigen G250,Renal cell carcinoma-associated antigen G250,CA IX抗体 |
| Swissprot | Q16790 |
| Source | Mouse |
| Reactivity | Human |
| Immunogen | Synthetic Peptide |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:1000-1:3000;IHC,,1:50-300;IF,,1:50-1:200; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. |
| Tissue specificity | Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. |
| Clone No | Clone:9B5 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. |
Images
Western Blot analysis of 1) Hela, 2) 293T cells using CA9 Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using CA9 Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human liver cancer tissue using CA9 Monoclonal Antibody at dilution of 1:200.
