CASP3 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70388
宿主: Rabbit
反应性: H,M
应用: IHC
Overview
| Synonyms | A830040C14Rik,Apopain,CASP-3,CASP3,CASP3,Casp3a,Caspase 3,Caspase 3,apoptosis-related cysteine peptidase,Caspase 3,apoptosis-related cysteine protease,Caspase 3,apoptosis-related cysteine protease a,Caspase-3 subunit p12,CC3,CPP-32,CPP32,CPP32B,Cysteine protease CPP32,EC 3.4.22.56,LICE,mldy,OTTHUMP00000165052,OTTHUMP00000165053,OTTHUMP00000165054,PARP cleavage protease,Procaspase3,Protein Yama,SCA 1,SCA-1,SREBP cleavage activity 1,Yama,CASP3抗体 |
| Swissprot | P70677,P42574,P55213 |
| Source | Rabbit |
| Reactivity | Human,Mouse |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse caspase3 |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:200-1:800 |
| Concentration | 500 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. |
| Tissue specificity | Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.Highest expression level in cerebral cortex. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3. |
Images
Immunohistochemistry analysis of paraffin-embedded A549 in Human breast using CASP3 Polyclonal Antibody at dilution of 1:400.
Immunohistochemistry analysis of paraffin-embedded A549 in Mouse lung using CASP3 Polyclonal Antibody at dilution of 1:400.
Immunohistochemistry analysis of paraffin-embedded A549 in Mouse Subcutaneous tumor using CASP3 Polyclonal Antibody at dilution of 1:400.
