CK-7 Polyclonal Antibody
| 规格： | / 50μL / 100μL |
| 价格： | / ￥1010 / ￥1640 |
货号：D-AB-10175L
货期：咨询
宿主: Rabbit
反应性: H,M
应用: WB,IHC
Overview
| Synonyms | CK 7, Cytokeratin 7, D15Wsu77e, K2C7, K7, Keratin 7, Keratin type II cytoskeletal 7, Krt2-7, MGC11625, Sarcolectin, Type II mesothelial keratin K7 |
| Swissprot | Q9DCV7 |
| Source | Rabbit |
| Reactivity | Human,Mouse |
| Immunogen | Recombinant Mouse Krt7 protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:250-1:500; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm |
| Tissue specificity | Expressed in most simple epithelia tested including liver, lactating mammary gland, lung, kidney, stomach, duodenum, colon, oviduct, uterus, pancreas, epididymis, prostate, preputial gland and mesothelium, and in most stratified epithelia tested including dorsal skin, paw/toe, tail, tongue, cervix, forestomach, and bladder. Also expressed in Henle layer of the inner root sheath of whisker follicle.Highest expression level in urinary bladder urothelium. |
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells,which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins,including K9–K23,and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins,including K1–K8,and the hair keratins,Hb1–Hb6. KRT7,also named as cytokeratin 7,is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels,and their neoplasms. KRT7 is marker of epithelial tissues,but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7. |
Images
Western Blot analysis of Mouse lung using CK-7 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of A431 and Hela cells using CK-7 Polyclonal Antibody at dilution of 1:2000
Western Blot analysis of A549 cells using CK-7 Polyclonal Antibody at dilution of 1:2000
Immunohistochemistry of paraffin-embedded Mouse colon using Krt7 Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Mouse kidney using Krt7 Polyclonal Antibody at dilution of 1:500
