CK-8 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70236
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | CARD2,CK 8,CK-8,CK8,CYK8,CYKER,Cytokeratin endo A,Cytokeratin-8,DreK8,EndoA,K2C8,K2C8,K8,Keratin 8,Keratin type II cytoskeletal 8,Keratin,type II cytoskeletal 8,Keratin-8,KO,Krt 2.8,KRT8,MGC118110,MGC174782,MGC53564,MGC85764,sb:cb186,Type-II keratin Kb8,CK-8抗体 |
| Swissprot | P05787,P11679,Q10758 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Cytokeratin 8 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:200-1:800; |
| Concentration | 430 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm |
| Tissue specificity | Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.Highest expression level in mucosa of transverse colon. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Cytokeratin 8 (CK8) has a molecular weight of 52.5 KDa, which belongs to the HMW (high molecular weight) B-type Cytokeratin. CK8 is mainly marked on non-squamous epithelium, thus can be used for the diagnosis of adenocarcinoma and ductal carcinoma. Generally, CK8 is not expressed in squamous cell carcinoma. It is reported that hepatocellular carcinoma mainly express CK8 and CK18. |
Images
Western Blot analysis of various samples using CK-8 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human colon using CK-8 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded human stomach cancer using CK-8 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded mouse lung using CK-8 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded rat stomach using CK-8 Polyclonal Antibody at dilution of 1:300.
