CKMT1A/CKMT1B Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-19301
宿主: Rabbit
反应性: H
应用: WB,IHC,ELISA
Overview
| Synonyms | Acidic-type mitochondrial creatine kinase,CKMT,CKMT1,CKMT1B,Creatine kinase mitochondrial 1 (ubiquitous),Creatine kinase mitochondrial 1B,Creatine kinase U type mitochondrial,Creatine kinase U-type,KCRU,Mia-CK,mitochondrial,U-MtCK,Ubiquitous mitochondrial creatine kinase,UMTCK,CKMT1A/CKMT1B抗体 |
| Swissprot | P12532 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Synthetic peptide of human CKMT1A/CKMT1B |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213),ELISA |
| Recommended dilution | WB 1:500-1:2000, IHC 1:40-1:200, ELISA 1:5000-1:10000 |
| Concentration | 1.68mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Mitochondrion inner membrane. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. |
Images
Western blot analysis of MCF7 cell lysate using CKMT1A/CKMT1B Polyclonal Antibody at dilution of 1:600
Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using CKMT1A/CKMT1B Polyclonal Antibody at dilution of 1:55(×200)
Immunohistochemistry of paraffin-embedded Human tonsil tissue using CKMT1A/CKMT1B Polyclonal Antibody at dilution of 1:55(×200)
