Crystallin-alpha B Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-10781
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | AACRYA,Alpha B crystallin,Alpha crystallin B chain,Alpha(B)-crystallin,Alpha-crystallin B chain,CRYA2,Cryab,CRYAB,Crystallin alpha B,Crystallin alpha polypeptide 2,CTPP2,Heat shock 20 kD like protein,Heat shock protein beta 5,Heat shock protein beta-5,HspB5,Renal carcinoma antigen NY REN 27,Renal carcinoma antigen NY-REN-27,Rosenthal fiber component,CRYAB抗体 |
| Swissprot | P02511 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human CRYAB |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000; |
| Concentration | 0.2 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
| Tissue specificity | Lens as well as other tissues. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. |
Images
Western Blot analysis of Mouse heart and Human chromaffin cell tumor tissue, Mouse muscle tissue using Crystallin-alpha B Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human esophagus cancer using Crystallin-alpha B Polyclonal Antibody at dilution of 1:25
Immunohistochemistry of paraffin-embedded Human thyroid cancer using Crystallin-alpha B Polyclonal Antibody at dilution of 1:25
