CUL4A Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-19279
宿主: Rabbit
反应性: H,M
应用: WB,IHC
Overview
| Synonyms | 2810470J21Rik,AW495282,CUL 4A,CUL-4A,Cul4a,Cul4a protein,CUL4A,Cullin-4A,MGC36573,MGC64071,CUL4A抗体 |
| Swissprot | Q13619 |
| Source | Rabbit |
| Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human CUL4A |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:50-1:300; |
| Concentration | 1.62mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus,nucleoplasm,Other locations:Cul4-RING E3 ubiquitin ligase complex,Cul4A-RING E3 ubiquitin ligase complex |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | Cullin proteins assemble a large number of RING E3 ubiquitin ligases,participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins,CUL4A (87 kDa) and CUL4B(104 kDa),have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases,DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol,suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. |
Images
Western blot analysis of 231 and HepG2 cell lysates using CUL4A Polyclonal Antibody at dilution of 1:600
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CUL4A Polyclonal Antibody at dilution of 1:55(×200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CUL4A Polyclonal Antibody at dilution of 1:55(×200)
