DNA-RNA Hybrid Monoclonal Antibody[S9.6]
| 规格： | / 25μL / 100μL |
| 价格： | / ￥798 / ￥2100 |
货号：E-AB-48028
货期：咨询
宿主: Mouse
反应性: All
应用: IF,ChIP
Overview
| Synonyms | RNA/DNA hybrid |
| Source | Mouse |
| Reactivity | All |
| Immunogen | ΦX174 bacteriophage-derived synthetic DNA/RNA |
| Application | IF,ChIP |
| Recommended dilution | IF,,1:200;ChIP,,1:50-1:500 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Clone No | S9.6 |
|---|---|
| Isotype | IgG2a |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol |
| Background | The DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA. The antibody has high affinity for DNA-RNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since R-loop sequence affects binding affinity. |
Images
Immunofluorescentanalysis of (4% PFA) fixed HeLa cells using DNA-RNA hybrid mouse monoclonal antibody [S9.6] at dilution of 1:500.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using anti-DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:500.
Chromatin immunoprecipitation analysis of HeLa cells genomic DNA(gDNA) using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:200.
