ENO1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-11010
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | 2 phospho D glycerate hydro lyase,2-phospho-D-glycerate hydro-lyase,Alpha enolase,Alpha enolase like 1,Alpha-enolase,C myc promoter binding protein,C-myc promoter-binding protein,EC 4.2.1.11,eno1,ENO1L1,ENOA,Enolase 1 (alpha),Enolase 1 (alpha) like 1,Enolase 1,Enolase alpha,MBP 1,MBP-1,MBP1,MBPB1,MPB 1,MPB-1,MPB1,MYC promoter binding protein 1,NNE,Non neural enolase,Non-neural enolase,Phosphopyruvate hydratase,Plasminogen binding protein,Plasminogen-binding protein,PPH,Tau crystallin,ENO1抗体 |
| Swissprot | P06733 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human ENO1 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:50-1:200; |
| Concentration | 0.6 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus and Cytoplasm. Cell membrane. Cytoplasm>myofibril>sarcomere>M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. |
| Tissue specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. |
Images
Western Blot analysis of Hela, A431 and hepG2 cell, Mouse brain and liver tissue, Mouse pancreas and Human fetal brain tissue using ENO1 Polyclonal Antibody at dilution of 1:700
Immunohistochemistry of paraffin-embedded Mouse brain using ENO1 Polyclonal Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ENO1 Polyclonal Antibody at dilution of 1:60
Immunohistochemistry of paraffin-embedded Human Kidney using ENO1 Polyclonal Antibody at dilution of 1:60(Elabscience® Product Detected by Lifespan).
