GAPDH Monoclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-48016
宿主: Mouse
反应性: H,M,R,B,C,G,Rb,Pg
应用: WB,IF
Overview
| Synonyms | aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component |
| Swissprot | Q5XJ10 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat ,Bovine,Caprine,Guinea pig,Rabbit,Porcine, |
| Immunogen | Recombinant human GAPDH protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304),IF |
| Recommended dilution | WB,,1:5000-10000; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm, Cytoskeleton, Nucleus |
| Isotype | IgG1 |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR. |
Images
Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000.
Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20µg per lane.
Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000.
Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate.
Lysates/proteins at 20µg per lane.
Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.
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