GFP Monoclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-48010
宿主: Mouse
反应性: All
应用: WB, IF,IP,FCM
Overview
| Synonyms | GFP,Green fluorescent protein |
| Swissprot | P42212 |
| Source | Mouse |
| Reactivity | All |
| Immunogen | Recombinant GFP protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304), IF,IP,FCM |
| Recommended dilution | WB,,1:100000-200000;IP,,3ug/sample;IF,,1:8000-16000;FCM,,0.2ug; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Isotype | IgG2a |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization. |
Images
Western blot of eGFP protein with anti-eGFP at dilution of 1:80000.
Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:8000.
IP Result of 293 cells transfected with GFP-Tag fusion protein, using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
1x10^6 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(E-AB-48010) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
