GFP Monoclonal Antibody
| 规格： | / 50μL / 100μL |
| 价格： | / ￥499 / ￥800 |
货号：E-AB-48043
宿主: Mouse
反应性: All
应用: WB, IF,IP
Overview
| Synonyms | GFP,Green fluorescent protein |
| Swissprot | P42212 |
| Source | Mouse |
| Reactivity | All |
| Immunogen | Recombinant GFP protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304), IF,IP |
| Recommended dilution | WB,,1:100000-200000;IF,,1:8000-16000;IP,,3ug/sample; |
| Concentration | 1mg/ml |
| Clonality | Monoclonal |
Properties
| Clone No | 2A7 |
|---|---|
| Isotype | IgG2a |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization. |
Images
Western blot of GFP protein with anti-GFP at dilution of 1:50000, 1:100000, 200000. Lane 1:293F cells transfected with GFP-Tag fusion protein, lane 2: 293F cells transfected with GFP-Tag fusion protein, Lane 3:293F cells transfected with GFP-Tag fusion protein
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:50000.
IP Result of 293F cells transfected with GFP-Tag fusion protein,using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
