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GFP Monoclonal Antibody

规格: / 30μL / 60μL / 120μL / 300μL / 500μL
价格: / ¥295 / ¥548 / ¥875 / ¥1975 / ¥2835

货号:E-AB-20086

宿主: Mouse

应用: WB,IP

  • 详情
  • 文献(2)
  • Overview

    Synonyms GFP,Green fluorescent protein,GFP抗体
    Source Mouse
    Immunogen Recombinant Protein
    Application WB(Detection kit: E-IR-R304),IP
    Recommended dilution WB 1:5000-1:10000, IP 1:100-1:300
    Concentration 1mg/mL
    Clonality Monoclonal

    Properties

    Clone No Clone:3I3
    Isotype IgG
    Purification Protein A purification
    Conjugation Unconjugated
    Storage instructions Store at -20℃. Avoid freeze / thaw cycles.
    Storage buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.4
    Background Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).
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