GLS Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-19340
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | AAD20,DKFZp686O15119,FLJ10358,GAC,GAM,GLS,GLS1,GLSK,Glutaminase C,Glutaminase kidney isoform,Glutaminase phosphate activated,K-glutaminase,KGA,KIAA0838,L-glutamine amidohydrolase,mitochondrial,GLS抗体 |
| Swissprot | O94925 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human GLS |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:50-1:200; |
| Concentration | 1.02mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Mitochondrion. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | GLS,also named as GLS1 and KIAA0838,belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-,glutamate-,and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells,in accordance with measurements of glutaminase activity,and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase. It has 3 isoforms named as KGA,GAM,GAC. |
Images
Western blot analysis of 293T cell lysate using GLS Polyclonal Antibody at dilution of 1:850
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using GLS Polyclonal Antibody at dilution of 1:70(×200)
Immunohistochemistry of paraffin-embedded Human tonsil tissue using GLS Polyclonal Antibody at dilution of 1:70(×200)
