HIF1 alpha Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-31662
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC-p,IF,ELISA
Overview
| Synonyms | ARNT interacting protein,ARNT-interacting protein,Basic helix loop helix PAS protein MOP1,Basic-helix-loop-helix-PAS protein MOP1,bHLHe78,Class E basic helix-loop-helix protein 78,HIF 1A,HIF 1alpha,HIF-1-alpha,HIF1 A,HIF1 Alpha,HIF1,HIF1-alpha,HIF1A,HIF1A,Hypoxia inducible factor 1 alpha,Hypoxia inducible factor 1 alpha isoform I.3,Hypoxia inducible factor 1 alpha subunit,Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor,Hypoxia inducible factor 1,alpha subunit (basic helix loop helix transcription factor),Hypoxia inducible factor1alpha,Hypoxia-inducible factor 1-alpha,Member of PAS protein 1,Member of PAS superfamily 1,Member of the PAS Superfamily 1,MOP 1,MOP1,PAS domain-containing protein 8,PASD 8,PASD8,HIF-1α抗体 |
| Swissprot | Q16665 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the Internal region of human HIF-1α. |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF,ELISA |
| Recommended dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000 |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. |
| Tissue specificity | Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. |
Images
Western Blot analysis of HeLa cells using HIF1 alpha Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using HIF1 alpha Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse lung tissue using HIF1 alpha Polyclonal Antibody at dilution of 1:200.
