His-Tag Monoclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-48003
宿主: Mouse
反应性: All
应用: WB,IP,FCM
Overview
| Synonyms | 6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag, Polyhistidine Tag |
| Source | Mouse |
| Reactivity | All |
| Immunogen | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Application | WB(Detection kit: E-IR-R304),IP,FCM |
| Recommended dilution | WB,,1:1000-2000;IP,,3ug/sample;FCM,,0.2ug; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Isotype | IgG1 |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world. |
Images
Western blot of His-Tag fused recombinant protein with Anti-His mouse monoclonal antibody at dilution of 1:1000.
IP Result of 293F cells transfected with His-Tag fusion protein,using anti-His-Tag mouse monoclonal antibody . Lane 1:input, Lane 2: anti-His-Tag mouse monoclonal antibody, Lane 3: mouse IgG1 Isotype Control
1x106 CHO cells Transfected with a His plasmid were stained with 0.2ug Anti-His tag mAb(E-AB-48003) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
