HMGB1 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70044
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC,IF
Overview
| Synonyms | Amphoterin,Chromosomal protein,nonhistone,HMG1,DKFZp686A04236,High mobility group 1,High mobility group box 1,High mobility group protein 1,High mobility group protein B1,high-mobility group (nonhistone chromosomal) protein 1,HMG-1,HMG1,HMG3,HMGB 1,HMGB1,HMGB1,NONHISTONE CHROMOSOMAL PROTEIN HMG1,SBP 1,Sulfoglucuronyl carbohydrate binding protein,HMGB1抗体 |
| Swissprot | P09429,P63158,P63159 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse HMGB1 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:200-1:800;IF,,1:200-1:800; |
| Concentration | 1.45 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane. Endosome. Endoplasmic reticulum-Golgi intermediate compartment. In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118). |
| Tissue specificity | Ubiquituous. Expressed in platelets (PubMed:11154118).Highest expression level in kidney. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin.Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix.HMG proteins synergize with Oct-2, members of the NF B family, ATF-2 and c-Jun to activate transcription.Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein.Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes.HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone. |
Images
Western Blot analysis of various samples using HMGB1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using HMGB1 Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using HMGB1 Polyclonal Antibody at dilution of 1:500.
Immunofluorescence analysis of paraffin-embedded Mouse heart using HMGB1 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded Human Tonsil using HMGB1 Polyclonal Antibody(Elabscience® Product Detected by Lifespan).
