HO1/HMOX1 Polyclonal Antibody
| 规格： | / 25μg / 100μg |
| 价格： | / ￥800 / ￥2100 |
货号：AN000380P
货期：咨询
宿主: Rabbit
反应性: H;M
应用: WB;IHC;ELISA Capture/Detector
Overview
| Synonyms | Heme oxygenase 1;HO-1;HMOX1;HO;HO1;EC:1.14.14.18 |
| Swissprot | P09601 |
| Source | Rabbit |
| Reactivity | Human;Mouse |
| Immunogen | Recombinant Human HO1/HMOX1 protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304);IHC(Detection kit: E-IR-R213);ELISA Capture/Detector |
| Recommended dilution | WB,,1:500-1:1000;IHC,,1:500-1:1000;ELISA Capture,,2-8 μg/mL;ELISA Detector,,0.1-0.4 μg/mL |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C Valid for 1 month or -20°C Valid for 12 months,Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS,pH 7.4 |
| Background | Heme Oxygenase (HO),also known as HMOX,is the rate limiting enzyme in the breakdown of heme into Biliverdin,CO,and iron. There are two major isoforms of this enzyme,HO-1 and HO-2. Both have similar enzymatic activity,however,HO-1 is rapidly inducible,while HO-2 is constitutively expressed. Inducers of HO-1 include cell stressors such as oxidative stress,infection,hypoxia,and cytotoxic agents. The reaction products generated by HO-1 activity are biologically active,and the enzyme has a broad range of putative roles. For instance,Biliverdin is an antioxidant with cytoprotective and anti-inflammatory properties,and CO has the potential to suppress inflammation and apoptosis. Because of these activities,HO-1 has received much attention as a therapeutic target for a range of pathological processes. |
Images
Western Blot with HO1 Polyclonal Antibody at dilution of 1:500.Lane 1:A549 cell lysate,Lane 2:NIH-3T3 cell lysate
Immunohistochemistry of paraffin-embedded Human breast cancer using HO1 Polyclonal Antibody at dilution of 1:1000
Sandwich ELISA-Recombinant Human HO1/HMOX1 protein standard curve.Background subtracted standard curve using HO1/HMOX1 antibody(AN000380P)(Capture),HO1/HMOX1 antibody(AN000380P)(Detector) in sandwich ELISA.The reference range value for Recombinant Human HO1/HMOX1 protein is 0.31-20 ng/mL.
