HRP-conjugated PCNA Monoclonal Antibody
| 规格： | / 25μL / 100μL |
| 价格： | / ￥350 / ￥920 |
货号：AN00472HP
货期：咨询
宿主: Mouse
反应性: H;M;R
应用: WB
Overview
| Synonyms | Proliferating cell nuclear antigen,PCNA,Cyclin |
| Swissprot | P12004 |
| Source | Mouse |
| Reactivity | Human;Mouse;Rat |
| Immunogen | Recombinant human PCNA protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304) |
| Recommended dilution | WB,,1:2500-1:5000 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Clone No | Da-9C6 |
|---|---|
| Isotype | IgG2a |
| Purification | Protein A/G Purification |
| Conjugation | HRP |
| Storage instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Storage buffer | PBS with 0.1% procline 300,1% protective protein and 50% glycerol,pH7.4 |
| Background | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
