HSP40-4 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-11313
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | DJ 2,DJ2,DjA1,DnaJ (Hsp40) homolog,subfamily A,member 1,DnaJ homolog subfamily A member 1,DnaJ protein homolog 2,DNAJ2,Dnaja1,DNJA1,hDJ 2,HDJ-2,HDJ2,Heat shock 40 kDa protein 4,heat shock protein DNAJ like 2,Heat shock protein J2,HSDJ,HSJ-2,HSJ2,HSPF4,Human DnaJ protein 2,NEDD7,Neural precursor cell expressed developmentally down regulated 7,OTTHUMP00000021193,DNAJA1抗体 |
| Swissprot | P31689 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human DNAJA1 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:300; |
| Concentration | 0.5 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Membrane. |
| Tissue specificity | Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | DnaJ-like proteins interact with HSP 70 molecular chaperones and function to facilitate protein folding and mitochondrial protein import. HSP 40-4, also known as HDJ2, is the human DnaJ homolog that functions as a co-chaperone with a cysteine-rich zinc finger domain. The cellular redox enzyme thioredoxin interacts with HSP 40-4, and oxidation and reduction reversibly regulate HSP 40-4 function in response to the changing redox states of the cell. The zinc finger domain of HSP 40-4 may act as a redox sensor of chaperone-mediated protein-folding machinery, since HSP 40-4 inactivation leads to the oxidation of cysteine thiols and a simultaneous release of coordinated zinc. Loss of the HSP 40-4 protein may be linked to severe defects in spermatogenesis that involve aberrant androgen signaling. |
Images
Western Blot analysis of HepG2, Raji, A431 and 231 cell using HSP40-4 Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human colon cancer using HSP40-4 Polyclonal Antibody at dilution of 1:60
Immunohistochemistry of paraffin-embedded Human thyroid cancer using HSP40-4 Polyclonal Antibody at dilution of 1:60
