Ki67 Polyclonal Antibody
| 规格： | / 25μL / 100μL |
| 价格： | / ￥800 / ￥2100 |
货号：AN004340L
货期：咨询
宿主: Rabbit
反应性: H;M;R
应用: IHC
Overview
| Synonyms | MIB;MKI;marker of proliferation Ki;Proliferation marker protein Ki;Antigen identified by monoclonal antibody Ki;PPP1R;MKI67;KIA;MIB-;MIB-1;PPP1R105;marker of proliferation Ki-67;Ki67;Antigen identified by monoclonal antibody Ki-67;Antigen Ki67;Antigen KI-67;Proliferation marker protein Ki-67;Antigen identified by monoclonal Ki 67;Antigen identified by monoclonal Ki-67;MIB 1;Proliferation related Ki 67 antigen;Protein phosphatase 1 regulatory subunit 105;RP11-380J17.2 |
| Swissprot | P46013 |
| Source | Rabbit |
| Reactivity | Human;Mouse;Rat |
| Immunogen | Recombinant Human Ki67 protein expressed by E.coli |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:500-1:1000 |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus |
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Storage buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
| Background | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). |
Images
Immunohistochemistry of paraffin-embedded human small intestine using Ki67 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human tonsil using Ki67 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded mouse testis using Ki67 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded mouse ovary using Ki67 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded rat ovary using Ki67 Polyclonal Antibody at dilution of 1:1000.
