Lactate Dehydrogenase (LDH) Activity Assay Kit
乳酸脱氢酶(LDH)比色法测试盒
| 规格： | / 100 assays(43 samples) |
| 价格： | / ￥320 |
货号：E-BC-K046-S
检测仪器及波长: 紫外-可见分光光度计(450 nm)
样本类型: 血清;血浆;组织;细胞等样本
检测原理
以辅酶I为递氢体，LDH催化乳酸产生丙酮酸，丙酮酸与2,4-二硝基苯肼作用生成丙酮酸二硝基苯腙，后者在碱性溶液中呈棕红色，颜色的深浅与丙酮酸的浓度呈正比，通过测定OD值，可计算LDH的活力。
关键信息
| 指标简称 | LDH |
| 样本类型 | 血清;血浆;组织;细胞等样本 |
| 灵敏度 | 4 U/L |
| 检测范围 | 4-400 U/L |
| 精密度 | 批间差：7.2%批内差：2.2% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3例预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的检测范围(4-400 U/L)处理样本，请参考下表稀释：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
10-15 |
大鼠血清 |
20-30 |
|
人血浆 |
10-15 |
大鼠血浆 |
20-30 |
|
猪血清 |
20-30 |
10%小鼠肾组织 |
500-800 |
|
10%大鼠肝组织 |
800-1000 |
10%大鼠肺组织 |
300-500 |
注：稀释液为生理盐水(0.9% NaCl)或PBS (0.01 M，pH 7.4)。
客户发表文献
- 文献: Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway
样品类型: pig testicular cell (ST cell) - 文献: Leonurine Ameliorates Oxidative Stress and Insufficient Angiogenesis by Regulating the PI3K/Akt-eNOS Signaling Pathway in H2O2-Induced HUVECs
样品类型: HUVECs - 文献: A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation
样品类型: serum - 文献: Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice
样品类型: livers - 文献: Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota
样品类型: serum - 文献: CD137 Signal Mediates Cardiac Ischemia–Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes
样品类型: serum,HL-1 cell - 文献: Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice
样品类型: serum - 文献: Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence
样品类型: serum - 文献: Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats
样品类型: testis - 文献: miR‐129‐5p attenuates hypoxia‐induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy
样品类型: H9c2 cell - 文献: Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway
样品类型: serum - 文献: Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats
样品类型: serum - 文献: Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis
样品类型: serum - 文献: Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats
样品类型: Serum - 文献: Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease
样品类型: SH-SY5Y cell - 文献: Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer:
样品类型: MC3T3-E1 cells - 文献: San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress
样品类型: hepatic tissue,serum - 文献: Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains
样品类型: brain - 文献: Protective effects of ghrelin against oxidative stress, inducible nitric oxide synthase and inflammation in a mouse model of myocardial ischemia/reperfusion injury via the HMGB1 and TLR4/NF-κB pathway
样品类型: serum - 文献: Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin
样品类型: plasma
