LMNA Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70326
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | 70 kDa lamin,Cardiomyopathy dilated 1A (autosomal dominant),CDCD1,CDDC,CMD1A,CMT2B1,EMD2,FPL,FPLD,FPLD2,HGPS,IDC,Lamin A,Lamin A/C,Lamin A/C like 1,Lamin,Lamin C,Lamin-A/C,LDP1,LFP,LGMD1B,Limb girdle muscular dystrophy 1B (autosomal dominant),LMN 1,LMN A,LMN C,LMN1,LMNA,LMNA,LMNC,LMNL1,Prelamin A/C,PRO1,Renal carcinoma antigen NY REN 32,Renal carcinoma antigen NY-REN-32,Renal carcinoma antigen NYREN32,LMNA抗体 |
| Swissprot | P02545,P48678,P48679 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Lamin A/C |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:1000-1:2000;IHC,,1:300-1:800; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Nucleus envelope. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localization of non-farnesylated prelamin-A/C. |
| Tissue specificity | Expressed in liver and in bone marrow (at protein level). Isoform C2 is specifically expressed in germ cells. Expressed in cardiomyocytes.Highest expression level in ascending aorta. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. |
Images
Western Blot analysis of various samples using LMNA Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using LMNA Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using LMNA Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded mouse liver using LMNA Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded rat liver using LMNA Polyclonal Antibody at dilution of 1:300.
