MAP1LC3A Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22136
宿主: Mouse
反应性: H,M,R
应用: WB,IHC-p,IF
Overview
| Synonyms | ATG8E,Autophagy-related protein LC3 A,Autophagy-related ubiquitin-like modifier LC3 A,LC3,LC3A,MAP1 light chain 3 like protein 1,MAP1 light chain 3-like protein 1,MAP1A/1B light chain 3 A,MAP1A/MAP1B LC3 A,MAP1A/MAP1B light chain 3 A,MAP1ALC3,MAP1BLC3,Map1lc3a,Microtubule associated proteins 1A/1B light chain 3,Microtubule-associated protein 1 light chain 3 alpha,Microtubule-associated proteins 1A and 1B,light chain 3,Microtubule-associated proteins 1A/1B light chain 3A,MLP3A,LC3A Mouse (5G10)抗体 |
| Swissprot | Q9H492 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of LC3A |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:200;IF,,1:100-1:300; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm>cytoskeleton. Endomembrane system. Cytoplasmic vesicle>autophagosome membrane. LC3-II binds to the autophagic membranes. |
| Tissue specificity | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. |
| Clone No | Clone:7E4 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. |
Images
Western Blot analysis of 1) Hela, 2) 3T3, 3) Rat brain using MAP1LC3A Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat kidney tissue using MAP1LC3A Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse spleen tissue using MAP1LC3A Monoclonal Antibody at dilution of 1:200.
