MCM3 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-40230
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | Cervical cancer proto oncogene 5,DNA polymerase alpha holoenzyme associated P1,DNA polymerase alpha holoenzyme associated protein P1,DNA polymerase alpha holoenzyme-associated protein P1,DNA replication factor MCM3,DNA replication licensing factor mcm3,HCC 5,HCC5,hRlf beta subunit,Human cervical cancer proto oncogene 5,MCM 3,mcm3,MCM3 minichromosome maintenance deficient 3,MCM3,MGC1157,Minichromosome maintenance complex component 3,Minichromosome maintenance deficient 3,Minichromosome maintenance protein 3,P1 h,P1 MCM3,P1 Protein,P1-MCM3,P1.h,p102,P102 protein,Replication licensing factor beta subunit,RLF beta subunit,RLF subunit beta,RLFB,MCM3抗体 |
| Swissprot | P25205 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Human DNA replication licensing factor MCM3 protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:1000;IHC,,1:50-1:200; |
| Concentration | 2mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Chromosome, Nucleus |
| Tissue specificity | Highest expression level in embryo |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with mCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. |
Images
Western Blot analysis of HepG2 cell using MCM3 Polyclonal Antibody at dilution of 1:500
Western blotting with anti-MCM3 polyclonal antibody at dilution of 1:1000. Lane 1:PC-12 cell lysate,Lane 2:C6 cell lysate,lane 3:Raw264.7 cell lysate,lane 4:NIH/3T3 cell lysate.
Immunohistochemistry of paraffin-embedded Human lymph node using MCM3 Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human tonsil using MCM3 Polyclonal Antibody at dilution of 1:200(Elabscience® Product Detected by Lifespan).
