MDC1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-12802
宿主: Rabbit
反应性: H
应用: IHC
Overview
| Synonyms | Homologue to Drosophila photoreceptor protein calphotin,MDC 1,Mdc1,MDC1,Mediation of DNA damage checkpoint 1,Mediator of DNA damage checkpoint 1,Mediator of DNA damage checkpoint protein 1,NFBD 1,NFBD1,Nuclear factor with BRCT domains 1,Nuclear Factor with BRCT Domains Protein 1,MDC1抗体 |
| Swissprot | Q14676 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Synthetic peptide of human MDC1 |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:100-1:300 |
| Concentration | 1.2 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AFX. Colocalizes with APTX at sites of DNA double-strand breaks. |
| Tissue specificity | Highly expressed in testis. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. |
