Mouse Aβ1-40(Amyloid Beta 1-40) ELISA Kit
小鼠β淀粉样蛋白1-40(Aβ1-40)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2600 / ￥1800 / ￥11050 |
货号：E-EL-M3009
反应性: 小鼠
检测范围: 7.81~500 pg/mL
灵敏度: 4.69 pg/mL
用途
该试剂盒用于体外定量检测小鼠serum, plasma and other biological fluids中Aβ1-40浓度
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗小鼠Aβ1-40抗体包被于酶标板上，实验时样品或标准品中的小鼠Aβ1-40会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗小鼠Aβ1-40抗体和辣根过氧化物酶标记的亲和素。抗小鼠Aβ1-40抗体与结合在包被抗体上的小鼠Aβ1-40结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，Aβ1-40浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Aβ1-40的浓度。
| 反应类型 | 夹心法 |
| 规格 | 96T |
| 反应时间 | 3.5h |
| 反应性 | 小鼠 |
| 检测方法 | Colormetric |
| 检测范围 | 7.81—500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100μL |
| 样本类型 | serum, plasma and other biological fluids |
特异性
可检测样本中的小鼠Aβ1-40，且与其它相关蛋白无明显交叉反应。
重复性
板内，板间变异系数均<10%。
典型数据
由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | O.D | Average | Corrected |
|---|---|---|---|
| 500 | 2.451 2.45 |
2.451 | 2.392 |
| 250 | 1.699 1.609 |
1.654 | 1.595 |
| 125 | 0.945 0.973 |
0.959 | 0.9 |
| 62.5 | 0.495 0.471 |
0.483 | 0.424 |
| 31.25 | 0.275 0.273 |
0.274 | 0.215 |
| 15.63 | 0.18 0.172 |
0.176 | 0.117 |
| 7.81 | 0.118 0.116 |
0.117 | 0.058 |
| 0 | 0.061 0.057 |
0.059 | -- |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) |
21.67 | 58.10 | 224.59 | 24.26 | 60.84 | 202.07 |
| Standard
deviation |
1.13 | 2.71 | 9.57 | 1.24 | 2.79 | 8.14 |
| C V (%) | 5.21 | 4.66 | 4.26 | 5.11 | 4.59 | 4.03 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 86-95 | 91 |
| EDTA plasma (n=5) | 89-102 | 97 |
| Cell culture media (n=5) | 98-109 | 101 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 96-107 | 98-110 | 94-104 |
| Average (%) | 102 | 103 | 101 | |
| 1:4 | Range (%) | 87-100 | 90-98 | 92-103 |
| Average (%) | 92 | 93 | 96 | |
| 1:8 | Range (%) | 87-98 | 95-108 | 100-109 |
| Average (%) | 94 | 102 | 103 | |
| 1:16 | Range (%) | 82-97 | 98-110 | 84-96 |
| Average (%) | 90 | 104 | 91 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周；如果一周以后才使用试剂盒，请拆开试剂盒按照下表中的条件分别保存各组分。
| 中文名称 | 英文名称 | 规格 | 保存条件 |
|---|---|---|---|
| ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃，可存放6个月 |
| 冻干标准品 | Reference Standard | 2 支 | |
| 浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
| 浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光)，可存放6个月 |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
| 生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
| 底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
| 反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
- 1. 酶标仪(450nm波长滤光片)
- 2. 高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3. 37℃恒温箱，双蒸水或去离子水
- 4. 吸水纸
操作步骤
|
|
1.对应板孔中加入100μL标准品工作液或样本，37℃孵育90分钟 |
| 2.弃掉板内液体后，立即加入100μL生物素化抗体工作液，37℃孵育60分钟 | |
| 3.弃掉板内液体，洗板3次 | |
| 4.每孔加入100μL HRP酶结合物工作液，37℃孵育30分钟，弃掉板内液体，洗板5次 | |
| 5.每孔加入90μL底物溶液，37℃孵育15分钟 | |
| 6.每孔加入50μL终止液 | |
| 7.立即在450nm波长下读数，处理数据 |
客户发表文献
- 文献: Activation of PPARA-mediated autophagy reduces Alzheimer disease-like pathology and cognitive decline in a murine model
样品类型: Tissue homogenate,Cell culture supernatant,Cell lysate - 文献: Apicidin attenuates memory deficits by reducing the Aβ load in APP/PS1 mice
样品类型: Brain tissue - 文献: Chk1 Inhibition Ameliorates Alzheimer’s Disease Pathogenesis and Cognitive Dysfunction Through CIP2A/PP2A Signaling
样品类型: brain tissue,hippocampus - 文献: AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice
样品类型: Hippocampus tissue - 文献: 3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy
样品类型: brain tissue - 文献: Moringa Oleifera Alleviates Aβ Burden and Improves Synaptic Plasticity and Cognitive Impairments in APP/PS1 Mice
样品类型: brain tissue - 文献: Cornuside Is a Potential Agent against Alzheimer’s Disease via Orchestration of Reactive Astrocytes
样品类型: cerebral tissue,BV2 cell - 文献: Camellia oil improves Aβ25-35-induced memory impairment by regulating the composition of the gut microbiota and lipid metabolism in mice
样品类型: brain - 文献: Tangeretin Inhibits BACE1 Activity and Attenuates Cognitive Impairments in AD Model Mice
样品类型: cortex - 文献: Corallodiscus flabellata B. L. Burtt extract and isonuomioside A ameliorate Aβ25−35-induced brain injury by inhibiting apoptosis, oxidative stress, and autophagy via the NMDAR2B/CamK Ⅱ/PKG pathway
样品类型: Brain tissue - 文献: Sulforaphane inhibits the production of Aβ partially through the activation of Nrf2-regulated oxidative stress
样品类型: N2a/APP cell - 文献: Platelets transport β-amyloid from the peripheral blood into the brain by destroying the blood-brain barrier to accelerate the process of Alzheimer's disease in mouse models
样品类型: Serum - 文献: Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice
样品类型: hippocampus tissue - 文献: Thymidine and 2′-deoxyuridine reduce microglial activation and improve oxidative stress damage by modulating glycolytic metabolism on the Aβ25-35-induced brain injury
样品类型: Brain tissue - 文献: The role of LRP1 in Aβ efflux transport across the blood-brain barrier and cognitive dysfunction in diabetes mellitus
样品类型: brain tissue - 文献: Eriodictyol and Homoeriodictyol Improve Memory Impairment in Aβ25–35-Induced Mice by Inhibiting the NLRP3 Inflammasome
样品类型: brain - 文献: Codonopsis pilosula polysaccharide attenuates Aβ toxicity and cognitive defects in APP/PS1 mice
样品类型: Tissue homogenate - 文献: Plasma amyloid beta level changes in aged mice with cognitive dysfunction following sevoflurane exposure
样品类型: plasma - 文献: Adenosine ameliorated Aβ25−35-induced brain injury through the inhibition of apoptosis and oxidative stress via an ERα pathway
样品类型: cerebral cortex tissue - 文献: Study of mitophagy and ATP-related metabolomics based on β-amyloid levels in Alzheimer's disease
样品类型: Cell lysate - 文献: The Down-Expression of ACE and IDE Exacerbates Exogenous Amyloid-β Neurotoxicity in CB2R –/– Mice
样品类型: Cell lysate - 文献: Mouse induced pluripotent stem cells-derived Alzheimer’s disease cerebral organoid culture and neural differentiation disorders
样品类型: iPSCs cells - 文献: Vascular endothelial growth factor alleviates mitochondrial dysfunction and suppression of mitochondrial biogenesis in models of Alzheimer’s disease
样品类型: brain - 文献: Resveratrol Abrogates Hypoxia-Induced Up-Regulation of Exosomal Amyloid-β Partially by Inhibiting CD147
样品类型: Tissue homogenate,Cell culture medium
