Myelin Basic protein Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3580 |
货号：E-AB-60400
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | MBP,MBP抗体 |
| Swissprot | P02686 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant fusion protein of human Myelin Basic protein (NP_001020271.1). |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,, 1:500-1:2000;IHC,, 1:50-1:200; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Myelin membrane. Cytoplasmic side of myelin. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Background | The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. |
Images
Western blot analysis of extracts of various cell lines using Myelin Basic protein Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat kidney using Myelin Basic protein Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using Myelin Basic protein Polyclonal Antibody at dilution of 1:200 (40x lens).
