N/H/K-Ras Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70313
宿主: Rabbit
反应性: M,R
应用: WB
Overview
| Synonyms | NRAS,HRAS1,GTPase NRas,Transforming protein N-Ras,HRAS,HRAS1,GTPase HRas,H-Ras-1,Ha-Ras,Transforming protein p21,c-H-ras,p21ras,KRAS,KRAS2,RASK2,GTPase KRas,K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras,N/H/K-Ras抗体 |
| Swissprot | P08644,P32883,Q61411,P20171 |
| Source | Rabbit |
| Reactivity | Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse K-Ras |
| Application | WB(Detection kit: E-IR-R304) |
| Recommended dilution | WB,,1:500-1:2000; |
| Concentration | 0.58 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cell membrane, Cytoplasm, Membrane |
| Tissue specificity | Highest expression level in lung |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | NRAS an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
