NPHS1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-12592
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | CNF,Nephrin,Nephrosis 1 congenital Finnish type,Nephrosis 1,congenital,Finnish type (nephrin),NPHN,NPHN,NPHS 1,Nphs1,Renal glomerulus specific cell adhesion receptor,Renal glomerulus-specific cell adhesion receptor,NPHS1抗体 |
| Swissprot | O60500 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human NPHS1 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:25-1:100; |
| Concentration | 0.5 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. |
| Tissue specificity | Specifically expressed in podocytes of kidney glomeruli. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates. |
