NPM1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-40477
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | B23, MGC104254, NO38, NPM, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), Nucleophosmin, nucleophosmin nucleoplasmin family member 1, Nucleophosmin/nucleoplasmin family member 1, Numatrin,NPM1抗体 |
| Swissprot | Q61937 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Mouse Nucleophosmin protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:10000-1:20000;IHC,,1:200-1:500; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm, Cytoskeleton, Nucleus |
| Tissue specificity | Expressed in B-cells that have been induced to switch to various Ig isotypes. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective,multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates,dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. |
Images
Western Blot analysis of 1)Hela, 2)293T, 3)A549, 4)Jurkat, 5)NIH/3T3, 6)PC-3, 7)Raji, 8)Caco-2 using NPM1 Polyclonal Antibody at dilution of 1:15000
Immunohistochemistry of paraffin-embedded Mouse colon using NPM1 Polycloanl Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Rat colon using NPM1 Polycloanl Antibody at dilution of 1:200.
