PDGFB Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70120
宿主: Rabbit
反应性: M,R
应用: WB,IHC
Overview
| Synonyms | Becaplermin,PDGF 2,PDGF B chain,PDGF subunit B,PDGF-2,PDGF2,Pdgfb,PDGFB,Platelet derived growth factor 2,Platelet-derived growth factor B chain,Platelet-derived growth factor beta polypeptide,Platelet-derived growth factor subunit B,Proto-oncogene c-Sis,SIS,SSV,PDGFB抗体 |
| Source | Rabbit |
| Reactivity | Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse PDGFB |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:200-1:400; |
| Concentration | 410 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Endoplasmic reticulum,endoplasmic reticulum lumen,Extracellular region or secreted,extracellular matrix,extracellular region,extracellular space,Golgi apparatus,Golgi lumen,Golgi membrane,Plasma Membrane,basolateral plasma membrane,Other locations:cell surface,cytoplasm,platelet alpha granule lumen |
| Tissue specificity | Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. |
Images
Western Blot analysis of various samples using PDGFB Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded mouse pancreas using PDGFB Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded Rat pancreas using PDGFB Polyclonal Antibody at dilution of 1:200.
