Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥630 / ￥1510 / ￥2415 |
货号：E-AB-20911
宿主: Rabbit
反应性: H,M,R,Mk
应用: WB,IHC-p,IF
Overview
| Synonyms | I kappa B alpha,I-kappa-B-alpha,IkappaBalpha,IkB-alpha,IKBA,IKBA,IKBalpha,MAD 3,MAD3,Major histocompatibility complex enhancer-binding protein MAD3,NF kappa B inhibitor alpha,NF-kappa-B inhibitor alpha,NFKBI,NFKBIA,Nuclear factor of kappa light chain gene enhancer in B cells,Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha,IκB-α (phospho Ser32/S36)磷酸化抗体 |
| Swissprot | P25963 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Synthesized peptide derived from human IκB-α around the phosphorylation site of Ser32/S36 |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:300;IF,,1:200-1:1000; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
Images
Western Blot analysis of COS-7 cells with Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody
Immunohistochemistry of paraffin-embedded Mouse kidney tissue using Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody at dilution of 1:200
Immunofluorescence analysis of Rat spleen tissue using Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody at dilution of 1:200
客户发表文献
- 文献: Targeting Nrf2 with 3 H-1,2-dithiole-3-thione to moderate OXPHOS-driven oxidative stress attenuates IL-17A-induced psoriasis
样品类型: human keratinocyte HaCaT cell - 文献: Ameliorating Effect of the Total Flavonoids of Morus nigra L. on Prediabetic Mice Based on Regulation of Inflammation and Insulin Sensitization
样品类型: liver,AML-12 cell - 文献: Trifluoperazine reduces cuprizone-induced demyelination via targeting Nrf2 and IKB in mice
样品类型: cortex - 文献: OPN N-glycosylation promoted bone destruction
样品类型: RAW264.7 cell,MC3T3-E1 cell - 文献: Dapsone reduced cuprizone-induced demyelination via targeting Nrf2 and IKB in C57BL/6 mice
样品类型: prefrontal cortex - 文献: Role of fish collagen hydrolysate in attenuating inflammation—An in vitro study
样品类型: RAW 264.7 macrophages - 文献: Renoprotective effects of prazosin on ischemia-reperfusion injury in rats:
样品类型: renal
