PI 3 kinase p85 alpha Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22164
宿主: Mouse
反应性: M,R
应用: WB,IHC-p
Overview
| Synonyms | GRB1,p85 alpha,p85,P85A,Phosphatidylinositol 3 kinase associated p 85 alpha,Phosphatidylinositol 3 kinase regulatory 1,Phosphatidylinositol 3 kinase,regulatory subunit,polypeptide 1 (p85 alpha),Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha,Phosphatidylinositol 3-kinase regulatory subunit alpha,Phosphoinositide 3 kinase,regulatory subunit 1 (alpha),PI3 kinase p85 subunit alpha,PI3-kinase regulatory subunit alpha,PI3-kinase subunit p85-alpha,PI3K,PI3K regulatory subunit alpha,Pik3r1,PtdIns 3 kinase p85 alpha,PtdIns-3-kinase regulatory subunit alpha,PtdIns-3-kinase regulatory subunit p85-alpha,PI3 Kinase P85α抗体 |
| Swissprot | P27986 |
| Source | Mouse |
| Reactivity | Mouse,Rat |
| Immunogen | Recombinant Protein |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:1000-2000;IHC,,1:100-200; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytosol,Endoplasmic reticulum,perinuclear endoplasmic reticulum membrane,Nucleus,Plasma Membrane,Other locations:cell-cell junction,cis-Golgi network,cytoplasm,membrane,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,protein complex |
| Tissue specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
| Isotype | IgG |
| Purification | Protein A purification |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4. |
| Background | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
Images
Western Blot analysis of 1) 3T3, 2) Rat liver using PI 3 kinase p85 alpha Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Rat liver tissue using PI 3 kinase p85 alpha Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Mouse brain tissue using PI 3 kinase p85 alpha Monoclonal Antibody at dilution of 1:200.
客户发表文献
- 文献: Secretory phosphoprotein 1 secreted by fibroblast-like synoviocytes promotes osteoclasts formation via PI3K/AKT signaling in collagen-induced arthritis
样品类型: synovial tissue,Fibroblast-like synoviocytes (FLSs)
