Purified Anti-Human CD45 Antibody[HI30]
| 规格： | / 25μg / 100μg |
| 价格： | / ￥160 / ￥400 |
货号：E-AB-F1137A
货期：咨询
宿主: Mouse
反应性: H
应用: FCM
Overview
| Synonyms | CD45;L-CA;Ly-5;Ptprc;Receptor-type tyrosine-protein phosphatase C;T200 |
| Swissprot | P08575 |
| Source | Mouse |
| Reactivity | Human |
| Immunogen | Recombinant Human CD45 protein |
| Application | FCM |
| Recommended dilution | FCM,,2 μg/mL(1×10^5-5×10^5 cells) |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Clone No | HI30 |
|---|---|
| Isotype | Mouse IgG1, κ |
| Purification | >98%, Protein A/G purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Storage buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
| Background | CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. |
