RACGAP1 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70169
宿主: Rabbit
反应性: M
应用: IHC
Overview
| Synonyms | CYK4,GAP,Gap1,GTPase activating protein,HsCYK-4,ID GAP,KIAA1478,Male germ cell RacGap,MgcRacGAP,Protein CYK4 homolg,Protein CYK4 homolog,Rac GTPase activating protein 1,Rac GTPase-activating protein 1,RACGAP 1,Racgap1,RGAP1,RACGAP1抗体 |
| Swissprot | Q9WVM1 |
| Source | Rabbit |
| Reactivity | Mouse |
| Immunogen | Recombinant protein corresponding to Mouse Racgap1 |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:200-1:800 |
| Concentration | 1.7 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Cytoplasm. Cytoplasm>cytoskeleton>spindle. Cytoplasmic vesicle>secretory vesicle>acrosome. Cleavage furrow. Midbody. Colocalizes with RND2 in Golgi-derived proacrosomal vesicles and the acrosome (By similarity). During interphase, localized to the nucleus and cytoplasm along with microtubules, in anaphase, is redistributed to the central spindle and, in telophase and cytokinesis, to the midbody. Colocalizes with RHOA at the myosin contractile ring during cytokinesis. Colocalizes with ECT2 to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalizes with Cdc42 to spindle microtubules from prometaphase to telophase. |
| Tissue specificity | Highly expressed in testis, thymus and spleen and weakly expressed in brain, heart, skeletal muscle and kidney. In testis, expression is restricted to germ cells with the Highest levels of expression found in spermatocytes. Not detected in adult liver. Also expressed in fetal liver and in several hematopoietic cell lines.Highest expression level in endocardial cushion. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Rac GTPase-activating protein 1 (RACGAP1),localized in the mitotic spindle,has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis,control of cell growth and differentiation,regulation of spermatogenesis,and tumor metastasis. |
