RBFOX3 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70267
宿主: Rabbit
反应性: M,R
应用: WB,IHC
Overview
| Synonyms | FLJ56884,FLJ58356,Fox-1 homolog C,fox1 homolog C,Fox3,FOX3NeuN,hexaribonucleotide binding protein 3,HRNBP3,NEUN,neuronal nuclei,Rbfox3,RFOX3,RNA binding protein fox-1 homolog 3,RNA binding protein,fox 1 homolog (C. elegans) 3,RBFOX3抗体 |
| Swissprot | Q8BIF2 |
| Source | Rabbit |
| Reactivity | Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to NeuN |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:300-1:800; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm, Nucleus |
| Tissue specificity | Widely expressed in brain, including in cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord (at protein level). Not expressed in all neuronal cells within a region, in cerebellum, expression is absent in Purkinje cells (at protein level). Expressed in the retina in the ganglion cells and some cells in the inner nuclear layer, but absent from the photoreceptor cells and most cells in the inner nuclear layer (at protein level).Highest expression level in lumbar subsegment of spinal cord. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism. |
Images
Western Blot analysis of various samples using RBFOX3 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded mouse brain using RBFOX3 Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry analysis of paraffin-embedded rat brain using RBFOX3 Polyclonal Antibody at dilution of 1:300.
