S100A9 Polyclonal Antibody(Capture/Detector)
| 规格： | / 25μg / 100μg |
| 价格： | / ￥800 / ￥2100 |
货号：AN000390P
货期：咨询
宿主: Rabbit
反应性: H
应用: ELISA Capture/Detector
Overview
| Synonyms | Protein S100-A9;CaLgranuLin-B; CaLprotectin L1H subunit; Leukocyte L1 compLex heavy chain;Migration inhibitory factor-reLated protein 14;MRP-14;p14;S100 caLcium-binding protein A9;S100A9;CAGB;CFAG;MRP14 |
| Swissprot | P06702 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Recombinant Human S100A9 protein expressed by E.coli |
| Application | ELISA Capture/Detector |
| Recommended dilution | ELISA Capture,,2-8 μg/mL;ELISA Detector,,0.1-0.4 μg/mL |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C Valid for 1 month or -20°C Valid for 12 months,Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS,pH 7.4 |
| Background | S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis,adhesion,can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK,PI3K/AKT,and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism,modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase . Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane,transferring arachidonic acid,an essential cofactor,to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory,antimicrobial,oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes,promotion of cytokine and chemokine production,and regulation of leukocyte adhesion and migration . Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TlR4) and receptor for advanced glycation endproducts (AGER). Binding to TlR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3 |
