SETDB1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-52933
宿主: Rabbit
反应性: H,M
应用: IHC
Overview
| Synonyms | AU022152,EC 2.1.1.43,ERG-associated protein with SET domain,ESET,H3 K9 HMTase 4,H3-K9-HMTase 4,H3-K9-HMTase4,Histone H3 K9 methyltransferase 4,Histone H3-K9 methyltransferase 4,Histone-lysine N-methyltransferase SETDB1,KG1T,KIAA0067,KMT1E,Lysine N-methyltransferase 1E,MGC90670,mKIAA0067,SET domain bifurcated 1,Set domain protein,bifurcated,1,SETB1,Setdb1,SETDB1抗体 |
| Swissprot | Q15047 |
| Source | Rabbit |
| Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human SETDB1 |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:50-1:300 |
| Concentration | 1.08mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | SETDB1,also named as ESET,KIAA0067 and KMT1E,belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1,CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions,thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation,gene silencing,and transcriptional repression. It has been identified as a target for treatment in Huntington Disease,given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. |
