SMAD7 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-53271
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | CRCS3,FLJ16482,hSMAD 7,hSMAD7,MAD (mothers against decapentaplegic Drosophila) homolog 7,MAD,Mad homolog 7,MAD homolog 8,MAD mothers against decapentaplegic homolog 7,MADH 7,MADH 8,MADH6,MADH8,Mothers Against Decapentaplegic Drosophila Homolog of 6,Mothers Against Decapentaplegic Drosophila Homolog of 7,Mothers against decapentaplegic homolog 7,Mothers against decapentaplegic homolog 8,Mothers against DPP homolog 7,Mothers against DPP homolog 8,SMA- AND MAD-RELATED PROTEIN 7,SMAD 7,SMAD,SMAD family member 7,SMAD,mothers against DPP homolog 7 (Drosophila),SMAD,mothers against DPP homolog 7,SMAD6,Smad7,SMAD7,SMAD7抗体 |
| Swissprot | O15105 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic peptide of human SMAD7 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:40-1:200; |
| Concentration | 1.14mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | SMAD7,also named as Mothers against decapentaplegic homolog 7,is a 426 amino acid protein,which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1,which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
Images
Western blot analysis of Mouse heart tissue lysate using SMAD7 Polyclonal Antibody at dilution of 1:900
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SMAD7 Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human tonsil tissue using SMAD7 Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Rat kidney using SMAD7 Polyclonal Antibody at dilution of 1:50
